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Objectives To investigate the correlation between HIF-1α expression level and clinical pathological characteristics of ovarian carcinoma in the elderly.Methods The expression levels of HIF-1α in normal ovary tissue,benign ovarian tumor tissue,borderline ovarian tumor tissue and ovarian cancer tissue were detected in the elderly by immunohistochemical method.And the correlations between HIF-1α expression level and clinical pathological characteristics of ovarian carcinoma were analyzed in the elderly.Results The HIF-1α expression was almost nothing in normal ovarian and benign ovarian tumor tissues,low in the borderline ovarian tissues,and high in the ovarian cancer tissue(Z=11.324,P =0.002).The IOD value of tissues was higher in borderline ovarian tumor tissue[(2.58 ± 0.65) × 105] and ovarian cancer tissue [(5.95 ± 1.02) × 105] than in normal ovary tissue[(1.90±0.35) × 105]and benign ovarian tumor tissue[1.95±0.41) × 105] (F=15.711,P=0.001).The expression of HIF-1α in the tissues was lower in ovarian cancer with stage Ⅰ-Ⅱ,high or moderate differentiation and non-metastatic lymph node than in ovarian cancer with stage Ⅲ-Ⅳ,low differentiation and metastatic lymph node (x2 =4.714,6.121,6.364;P =0.030,0.013,0.012).Conclusions The expression of HIF-1α is up-regulated in ovarian cancer in the elderly compared with normal ovarian tissues,which may be related to occurrence,development and clinical prognosis of ovarian cancer.
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Objective To detect the expression levels of serum alpha fetal protein (AFP),carbohydrate antigen 199(CA-199) and carcinoembryonic antigen(CEA) in a rat model of liver cancer and elderly liver cancer patients,and explore its clinical significance.Methods A rat model of liver cancer was successfully established by intraperitoneal injection of a low dose of diethylnitrosamine (DEN).The expression levels of serum AFP,CA-199 and CEA in rats were determined and compared during the development of liver cancer.Blood samples were collected from elderly subjects with normal liver and elderly liver cancer patients(n=80,each).The expressions of serum AFP,CA-199 and CEA were determined and compared between healthy elderly patients and elderly liver cancer patients.Results Serum levels of AFP,CA-199 and CEA were higher in liver cancer rats than in normal rats [(4.21±1.32) μg/Lvs.(1.05±0.33) μg/L,(3.78±1.04) kU/L vs.(1.00±0.28) kU/L,(3.54±0.92) μg/Lvs.(1.10±0.37) μg/L,t=9.493,8.609,7.675,respectively,all P=0.000].Serum AFP,CA-199 and CEA levels were higher in elderly patients with liver cancer than in elderly subjects with normal liver [(66.89±9.33) μg/L vs.(2.56±1.09) μg/L,(116.89±43.33) kU/L vs.(5.56±1.26) kU/L,(5.83 ± 1.56) μg/L vs.(1.17 ± 0.51) μg/L(t=14.379,17.470,10.677,respectively,all P=0.000).The positive rate of joint detection of AFP,CA-199 and CEA was higher in elderly patients with liver cancer than in elderly subjects with normal liver [77.50％ (62/80) vs.1.25％(1/80),x2 =17.260,P=0.000].Conclusions The joint detection of AFP,CA-199 and CEA can help increase the diagnostic rate of liver cancer in elderly patients.
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Excision repair cross complementing group 1 (ERCC1) gene is an enzyme of the speed limit of DNA repair protein in nucleotide excision repair (NER) pathways.Researches suggest that ERCC1 has been associated with cisplatin resistance in non-small cell lung cancer (NSCLC) patients.Thus,ERCC1 gene is a new target in malignant tumor gene therapy research,providing a new approach for the treatment of malignant,especially NSCLC.
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Matrix metallopmteinase 9(MMP-9) is one kind of matrix metalloproteinases and plays a role in the process of breast cancer metastasis. It can not only degrade the basement membrane and the extracellular matrix around tumor tissue but also promote angiogenesis, even create conditions for the growth of tumors, local invasion and distant metastasis. Current study shows that the over-expression of MMP-9 can significantly speed up the breast lymph node metastasis and the relevant research will provide guidance for clinical treatment and prognosis of breast cancer.
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<p><b>OBJECTIVE</b>To study the chemical constituents in the active portion from the flowers of Polygonum oriental.</p><p><b>METHOD</b>Chromatographic techniques were employed for isolation and purification of the constituents and their structures were determined by spectral analysis and chemical evidence.</p><p><b>RESULT</b>Nine compounds were obtained and identified as alphitonin (1), methyl 3, 4-dihydroxybenzoate (2), apocynin (3), kaempferol-3-O-beta-D-glucoside (4), 1,3,5-trihydroxybenzene (5), 3,3'-dimethoxyellagic-acid-4-O-beta-D-glucoside (6), kaempferol-3-O-alpha-L-rhamnoside (7), quercetin-3-O-alpha-L-rhamnoside (8), kaempferol (9).</p><p><b>CONCLUSION</b>Compounds 2, 4, 5 were isolated from P. oriental for the first time and compounds 1, 3 were firstly obtained from the genus Polygonum.</p>